STUDIES
Histologic Evaluation of Wound-Bed Preparedness Following Microsurfaced Skin Grafts for the Treatment of Deep Burn Wounds: Results from a Randomized Controlled Trial.
Lisa M. Marinelli MD; Kevin Krauland MD Department of Pathology and Area Laboratory Services, Brooke Army Medical Center, Fort Sam Houston, TX
Conclusion: Microsurfaced skin grafts resulted in consistent incorporation and greater cellular infiltration and graft thickness than their control graft counterparts, a finding we attribute to increased surface area at the graft-to-host interface.
Table 1. Histologic comparison of features of wound bed preparedness following microsurfaced and control split thickness skin grafts.
| Microsurfaced Split Thickness Skin Grafts | Control Split Thickness Skin Grafts | p-value | |
|---|---|---|---|
| Incorporation (Scale 1-5; median [IQR]) |
4 (4-5) |
2 (1-5) |
0.4 |
| Maximum (cell/mm2; median [IQR]) |
3,661 (1,966-6,403) |
1,511 (871-2258) |
0.004 |
| Predominant cell type of neutrophils (# [%]) |
9 (90) |
1 (10) |
0.001 |
| Total graft thickness (mm; mean [SD]) |
1.5 (0.82) |
0.92 (0.47) |
0.02 |
Porcine Study: David Stevens, DVM, PhD a
Premier Laboratory LLC, Longmont, CO
Clinical Hypothesis By resurfacing dermal grafts for internal reconstruction and external wounds you:
Increase the surface area and absorption of medications
Increased adhesion/seal between host - graft
Increase the cellular interaction between the graft and the human tissue
Faster incorporation of the resurfaced graft
Better fibrous foundation for healing
Histopathology Report Conclusions:
With respect to the dermal construct implants, the texturized samples elicited an enhanced inflammatory/cellular response compared to the controls, especially at day 7 and 14.
Texturizing amniotic membranes also enhanced the cellular response, especially in the day 7 samples.